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Cell Homogenization The pellets containing 3.55 10 6 cells were suspended in 0.1 M phosphate buffer with a pH of 7.5 (Sigma-Aldrich, Darmstadt, Germany), maintaining the proportion of 1 million cells to 0.04 mL of the buffer, then sonicated for 3 5 s at 4 C (Bandelin Sonoplus GM70, Berlin, Germany)

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With a molecular formula of CHNOS and a molecular weight of 9,117.5 g/mol (CAS: 946870-92-4), IGF-1 LR3 is supplied as a lyophilized powder for reconstitution in sterile water, ensuring optimal handling in controlled experimental settings
