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Panels show the Western analysis of elution products using immobilized GST-PRPF6N (amino acids 1306), GST-PRPF6M (amino acids 307607), and GST-PRPF6C (amino acids 607941)

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A serial dilution series of limiting concentrations of complexes were incubated with 2 nM of a radiolabelled target RNA, in 18 mM HEPES pH 7.4, 100 mM NaCl, 1 mM MgCl 2 , 2.5% (v/v) glycerol, 0.003% (v/v) IGEPAL CA-630, 0.5 mM BME, 0.017 mg ml 1 recombinant albumin and 0.003 mg ml 1 yeast tRNA, at 37 C for 1 h

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