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Total mtDNA copy number was determined using quantitative real-time PCR

The dissociation constants for titrations of Mdm2 against FAM-labeled 12/1 peptide were determined by fitting the experimental data to a 1:1 binding model equation shown below: [P] is the protein concentration (Mdm2), [L] is the labeled peptide concentration, r is the anisotropy measured, r 0 is the anisotropy of the free peptide, r b is the anisotropy of the Mdm2FAM-labeled peptide complex, K d is the dissociation constant, [L] t is the total FAM labeled peptide concentration, and [P] t is the total Mdm2 concentration

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In fact, some even claim that increasing your intake of vitamin B12 can help fight fatigue, boost energy levels, and speed up your metabolism