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The gradient started at 5% B (held for 2.5 min) and was linearly increased to 30% B within 37.5 min, to 50% B within 10 min, then to 85% B within 5 min (held for 3 min)

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Ribosomal protein-Mdm2-p53 pathway coordinates nutrient stress with lipid metabolism by regulating MCD and promoting fatty acid oxidation

For enzymatic assays in semen, the semen was diluted in an equal volume of Kiev solution and centrifuged for 20 min at 16000 g to separate spermatozoa and seminal fluid 89

It is used as an adjunct treatment to dietary and exercise measures to enhance glycemic control in adults diagnosed with type 2 diabetes mellitus [88]