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This novel probe might operate via a mechanism distinct from GSH , potentially opening up new possibilities for designing more potent GST blockers

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Cell pellets were later thawed and lysed with RLT Plus lysis buffer, and total RNA was extracted with the Qiagen RNeasy kit according to the manufacturers instructions (Qiagen, 74134)

Notably, Nrf2 plays a dual protective role by both repressing neuroinflammatory signaling and promoting the transcription of antioxidant genes, establishing it as a compelling therapeutic target in neurodegenerative disorders [182, 183]

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