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Beads were washed with 20 mM and 40 mM imidazole steps and proteins were eluted using 250 mM imidazole in 50 mM Tris-HCl, 100 mM NaCl, 0.5 mM MgCl 2 (pH 7.5)

It is sold as a research chemical NNMT inhibition has broad implications : NNMT is involved in multiple metabolic pathways beyond NAD+, including methylation balance, polyamine metabolism, and one-carbon metabolism

10.1113/JP285259 73

10.1016/s0140-6736(00)02490-9 Lancet (London, England)

De Souza JB, De Lacerda Coriolano D, Dos Santos Silva RC, Da Costa Jnior SD, De Almeida Campos LA, Cavalcanti IDL, Lira Nogueira MCDB, Pereira VRA, Brelaz-de-Castro MCA, Cavalcanti IMF

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