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To limit free metal concentrations, the synthesis of specific chelators and subsequent sequestration of metal complexes are of major importance

For four of these metabolites (homotaurine, N -acetyl-phenylalanylleucine/isoleucine, 3-hydroxypropane-1-sulfonic acid, and N 1 -methyl-imidazolelactic acid), the chemical standard did not match to the original tissue peak, but instead matched a peak with a distinct MS/MS spectrum and/or a retention time difference that could not be explained by chromatographic drift (see also Success rate of metabolite discovery)

, amperometry, voltammetry, electrochemical impedance spectroscopy, etc

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Plasma aldosterone levels and aldosterone-to-renin ratios are associated with endothelial dysfunction in young to middle-aged subjects

Similarly, a 24-week double-blind randomized trial in 80 patients with nonalcoholic simple fatty liver showed that curcumin supplementation significantly reduced hepatic fat content, body weight, and body mass index, with additional benefits on free fatty acids, triglycerides, fasting blood glucose, glycated hemoglobin, and insulin levels