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Simon P, Buta E, Gueorguieva R, Kong G, Morean ME, Camenga D , Bold KW, KrishnanSarin S

For organoids, RNA isolation was performed by removing 2 ml of suspension culture medium from the Erlenmeyer flasks and collecting the organoids by centrifugation at 300x g for 5 min at room temperature

References 1.DAlessandro A., Hansen K.C., Eisenmesser E.Z., Zimring J.C

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Furthermore, FRDA patient heart, liver, and spleen sections analyzed for iron content using Perls stain demonstrate extra-nuclear iron deposition, and in a chain-like organization, indicating mitochondrial localization ( Similarly, a FRDA mouse model of cardiac and skeletal muscle FXN loss demonstrates in cardiac tissue: increased TfR, decreased cytoplasmic ferritin heavy and light chain subunits, increased mitochondrial iron importer mitoferrin-2 (mfrn-2), and decreased expression of the iron exporter ferroportin (FPN) (Huang et al., 2009)

Nitric oxide stimulates growth hormone secretion from human fetal pituitaries and cultured pituitary adenomas