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The best number of components was calculated using a 10-fold cross-validation to minimize models error
10.7554/eLife.58147 [DOI] [PMC free article] [PubMed] [Google Scholar] Yasir M, Dutta D, Willcox MD

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Cells have a capability to keep track of the number of divisions undergone so that cell death can be initiated after a certain number of divisions

What matters more than the momentary discomfort is whether the program is administered appropriately and whether the patient fully understands why the injection is being used, what it is expected to do, and its medical limitations