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We then selected single transgenic lines that expressed comparable levels of each of the GSAAT1 variants in the homozygous gsa2 background ( Supplementary Figure 3A )

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The non-enzymatic succinylation is mainly mediated by intracellular succinyl-CoA which can be modulated by succinyl-CoA metabolism-targeting enzymes, such as OXCT1, a member of the CoA transferase family I [49], and SUCLA2/SUCLG2, the ADP-forming succinyl-CoA synthetase [32, 50]

To improve mitochondrial metabolism, studies explored boosting NAD + levels by administering NR to mdx mice, a DMD mouse model 254

Following the specified treatments, cells were collected and lysed and resulting global protein abundance was analyzed using LC-MS/MS

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