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RT-qPCR was performed with the LightCycler 480 system (Roche), following the manufacturer's instruction with SYBR Premix Ex Taq (TaKaRa)

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MK-777s innovation lies in potential improvements to receptor precision and tolerance though research is still in early stages

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Apart from clinical efficacy defined as the change in Hamilton Depression Rating Scale (HAMD)-17 score, secondary outcomes include changes in pathophysiological mechanisms related to depression as well as changes in local brain activity (functional Magnetic Resonance Imaging, fMRI) and white matter integrity (Diffusion Tensor Imaging, DTI)

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Iron chelators attenuate ischemia-reperfusion injury in animal models (Tuo et al., 2017), indicating that iron-induced ferroptosis may be the underlying mechanism of VD neuron loss

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Storage: Lyophilized: freeze at 20 C (4 F) for up to 12 months

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