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The crude extracts for the enzyme assay were prepared by homogenizing the tissue in 0.1 M MOPS buffer (Na 2-(N-morpholino) ethanesulfonate-0.1 M Na phosphate) pH 6.8

& Ni, Y

The enrichment of genes associated with regional emphysema severity with COPD-related phenotypes in these other datasets suggests that the biological processes associated with increasing emphysema severity within a patient with severe COPD also vary in individuals with earlier stages of disease

doi: 10.1186/1479-5876-10-224 16 RenJSamsonWKSowersJR

These include: Inflammatory bowel disease Coeliac Disease especially if the individual isnt following a strict gluten free diet Pancreatic insufficiency Pancreatic enzymes help to release vitamin B12 from the foods that we eat

For FixJ N and FixJ C the and folds were found, which is consistent with previously reported structural biological results on FixJ as a whole and of each domain