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After absorbance returned to baseline, the bound protein was eluted from the column with 100 mM maltose or 250 mM imidazole in lysis buffer
[DOI] [PMC free article] [PubMed] [Google Scholar] 207.Ooi S.L., Green R., Pak S.C
The reaction was allowed to proceed for 10 min, followed by cooling on ice
Metabolomics sample preparation Suspended cultures were harvested by transferring 25 mL aliquots into six conical tubes per sample and centrifugation at 4,300 g for 20 min at 4C
Impact of dietary habits, ethnicity, and geographical provenance in shaping human gut microbiome diversity
The highest content is in the United Kingdom, followed by Jordan and South Africa
