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In A, the linear sequence of amino acids 2235 is a functional cholesterol-binding domain that is unusual in that it does not contain the aromatic residues common in other cholesterol-binding protein domains

The cells were cultured in DMEM/F-12 medium (11320033, Gibco, USA) supplemented with 5% horse serum and 2.5% fetal bovine serum at 37C under an atmosphere of 5% CO 2

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