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To experimentally modulate arginase activity, specific inhibitors have been developed: N-hydroxy-L-arginine (NOHA) or N-hydroxy-nor-l-arginine (nor-NOHA), and boronic acid derivatives, such as 2(S)-amino-6-boronohexanoic acid (ABH), and S-(2-boronoethyl)-l-cysteine (BEC) s[229]

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Furthermore, following the same treatment protocol, the experiment was validated in 12-well plates (2 10 4 cells/well) with calcein/PI staining solution, and the ability of the GSH-CDs to rescue the NPCs was confirmed via fluorescence microscopy (Carl Zeiss, Germany)

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By combining these lifestyle habits with mindful nutrition, individuals can strengthen their natural defense system and maintain long-term cellular protection