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Over days of storage, bacteria multiply and produce endotoxins that could compromise your research results or cause adverse reactions
For genetic complementation, a DNA fragment containing 500 bp upstream of MSMEG_5827 (including its native promoter) was amplified and cloned into pHY plasmid via Gibson assembly to generate pHY- MSMEG_5827
The relative changes of PPAR- expression were quantified by ImageJ software (version 1.44, National Institutes of Health, Bethesda, USA)
R.YangP.BoothC
Furthermore, it has been observed that the greater the dietary fat consumed, the greater the likelihood of developing obesity [44]
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