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For further optimization, the central gene fragment of PapRS#0, flanked by the two Bsa I restriction sites, was subjected to error-prone PCR using the GeneMorph II random mutagenesis kit (Agilent), adjusting a mutagenesis frequency of 4 nucleotide exchanges

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Many studies have shown that high level of GPX2 promotes tumor growth, metastasis, and drug resistance, while reducing its expression helps inhibit tumor development [27, 55]

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Prolonged exposure to tobacco smoke, air pollution, pathogens and/or other risk factors submits lungs to a pro-oxidative environment, correlates with a functional decline of the lung epithelium 2,3,4 and can actively contribute to neoplastic transformation

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