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A CRISPR screen to identify redox vulnerabilities of KEAP1/NRF2-mutant tumors demonstrated hits in multiple enzymes in the pentose phosphate pathway, thioredoxin antioxidant system, glutathione reductase and mitochondrial superoxide dismutase 2 (SOD2) [78]

[DOI] [PubMed] [Google Scholar] 28.Frias Guerra L

and ( iii ) assembling into in FeS clusters, which still serve as electron conduits in contemporary biological redox reactions

The protein concentration in the samples was determined using Bradfords assay

Keep current-use vials refrigerated while maintaining longer-term inventory frozen

OD was recorded at 340 nm against blank