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Because NNMT activity has been associated with metabolic efficiency and cellular energy balance, researchers continue to explore how modulation of this enzyme may influence broader metabolic and biochemical pathways

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For in vitro experiments, embryonic rat cardiomyocytes (H9c2 cells) exposed to hypoxia-reoxygenation (HR) showed a time-dependent increase in gp91phox (a marker for ROS production by NADPH oxidases), caspase-3 (a key mediator of apoptosis) expression, and a decrease in the glutathione redox ratio