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Description
Luteolin protects against CIRI, potentially via regulation of the SIRT3/AMPK/mTOR signaling pathway

Select the chromatography method based on the difference in the properties of the target protein and the impurity protein: Ion Exchange Chromatography: Large difference in isoelectric points Hydrophobic Interaction Chromatography: Large differences in hydrophobicity Gel Filtration Chromatography: Large difference in molecular weight Multimodal Chromatography: Ion exchange and hydrophobic interaction chromatography alone cannot effectively separate Reversed-phase Chromatography: Generally not selected in protein purification, but can be selected in the purification of small molecule polypeptides Among them, ion exchange chromatography is convenient to operate and widely used

Luminescence was measured using a Glomax-Multi+ Luminometer (Promega)

Une carence en glutathion survient lorsque l'organisme ne produit plus suffisamment de cet antioxydant essentiel

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A further possible role of the ultra-rapid glutathionylation of a single cysteine is to limit the formation of non-natural disulfides, which may be more frequent in a disordered structure
