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Discriminating metabolite identification and exploratory biomarker analysis To identify metabolites contributing to the discrimination between ASD and control groups, feature-level statistical analyses were performed on selected metabolites derived from the untargeted LCMS dataset

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By employing genome-scale metabolic reconstruction and biodomain enrichment, the authors identified a core set of 62 genes and specific metabolite-gene pairings, such as MTHFR-homocysteine and PDHA1-acetyl-CoA, that drive pathological metabolic flux

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