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Prostate cancer (PCa) cell lines (PC-3, DU-145, LNCaP, 22Rv1) and benign prostate hyperplasia cell line (BPH) were purchased by American Type Culture Collection (ATCC) and cultured in RPMI, 10% Foetal Bovine Serum (FBS), 1% Glutamine, 1% PenStrept, at 5% CO 2 and 37 C