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Additional biochemical assays were used to test the ability of compounds to inhibit nicotinamide phosphoribosyl transferase (NAMPT) and NAD + -dependent protein deacetylase sirtuin 1 (SIRT1), two enzymes in the NAD + biosynthesis/salvage pathway

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After being rinsed thrice with TBS-T at 5-min intervals, the membranes were incubated with horseradish peroxidase-labeled goat anti-rabbit IgG (1:2000 dilution

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