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In some cases, possession or distribution of these peptides without proper authorization may result in legal consequences

doi: 10.1111/jne.12614 89 KellyMARubinsteinMAsaSLZhangGSaezCBunzowJRet al

Contribution of estrogen receptors alpha and beta to the effects of estradiol in the brain

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Once the correct sequence of all the three genes was confirmed, they were subcloned into pET28a (Novagen), bacterial expression vector and pYES2 (Invitrogen), yeast expression vector, under the control of galactose inducible and glucose repressible GAL1 promoter