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While the filtered GSH is degraded stepwise by the action of the brush-border enzymes -glutamyltransferase and cysteinylglycine dipeptidase, the remainder of the GSH appears to be transported via an unrelated, Na+-dependent system present in basal-lateral membranes

The cells were subcultured twice a week at a ratio of 1:5 to 1:8.Transient transfection of HeLa cells (12 10 5 cells/6-well) with pcDNA3.1(+) NAPstar or pSC2 HyPer7 (Addgene

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