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If you have a pH meter, leave the probe in your elution buffer with a stir bar rotating, and add one or two drops of concentrated sodium hydroxide at a time, then give the solution a few seconds for its pH to reach an equilibrium

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The oxidized product Di-NAC was quantified with freshly prepared Di-NAC standard the results are summarized in Table 6
Such mitochondria-targeted antioxidants include Mitoquinone (MitoQ), MitoVitE (vitamin E), MitoPBN (CoQ and phenyl tert-butyl nitrone conjugate), and MitoTEMPO (SOD mimetic)

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