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A Vitrobot Mark IV (FEI) operating at 4C, 100% humidity, wait time = 10 seconds, blot force = 4, blot time = 4 sec was used for blotting with grade 595 filter paper (Agar Scientific cat#AG47000) before plunging into liquid ethane
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Plewig and Kligman, 2000)
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Maintenance protocols: Chronic conditions (osteoarthritis) may benefit from ongoing low-dose treatment or periodic cycles
