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Reactive oxygen species (ROS), or free radicals, are byproducts of normal metabolism, produced constantly within the body

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It was also found that VIC exerts its antioxidant role not only by its action on ROS but also by redox regulation and enhancement of the activity of other cellular radical scavengers [48]

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Following extensive washing, the membranes were incubated with HRP-conjugated secondary antibodies (Thermo Fisher Scientific, USA)