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The completion rate of the everolimus group is lower due to side effects, and the incidence of grade 3 and 4 adverse events is higher

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Blots were blocked for 1 h in 5% non-fat milk in phosphate-buffered saline (PBS) with 0.05% Tween-20, and membranes were probed with primary antibodies overnight and horseradish-peroxidase-conjugated secondary antibodies (Bio-Rad, Hercules, CA, USA)

In apoptotic cells, DNA is cleaved into 180200 base pair fragments, the mitochondrial membrane potential decreases significantly, phosphatidylserine flips from the inner to the outer membrane, and no endolysosomal release or inflammatory response occurs (119)

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