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Over time, this chronic state of defensiveness may harden cell membranes, stop cells from communicating with each other, and cause cells to release ATP as a continued signal of cellular distress 2

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Initiation of pentostatin therapy in such patients can result in severe myelosuppression

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Cells were washed in PBS and fixed in solution using 4% paraformaldehyde and 0.0075% glutaraldehyde in PBS for 25 minutes
