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Finally, the cells were incubated with a nuclear staining solution (DAPI, 1:1000) for 5 min at RT

Greif, M

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Zinc, which is essential for the formation of myelin and can induce oxidative stress in its own right, may be involved in the abnormal homeostasis of bioactive metals, which might play a role in Alzheimer's disease - zinc binds to amyloid precursor protein 57 and aluminium, zinc, iron, and copper directly bind to amyloid protein promoting its aggregation

At this time, ROS affects spermatogenesis by regulating the receptor-tyrosine kinase (RTK) mediated pathway ( Figure 1 )