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For protein collection, the column was then inverted and centrifuged into a fresh tube, 1,000 g for 2 min

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0.16 ng/ml/12 ml/day till the sacrifice) and intragastrically after cuprizone instilations, while controls received simultaneously drinking water only (12 ml/day) as well as an equivolume of saline (5 ml/kg) intragastrically after cuprizone instillations

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