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References Chang, Chung-Hsun et al

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RNA extraction and RTqPCR Total RNA from cells was isolated via TRIzol (Takara, Otsu, Japan) and subsequently purified with chloroform/isopropanol/ethanol, and cDNA was synthesized via the PrimeScript RT reagent Kit (Takara) according to the manufacturers instructions and used as the template for qRTPCR

Naturally found in foods such as meats, fish, eggs, dairy, beans, and quinoa, amino acids are essential for maintaining optimal bodily function

doi: 10.1016/s0008-6363(99)00094-2

Available from: Accessed 2 Feb 2021 Hughes GB (1990) Practical management of Bells palsy