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Low levels of B12 can lead to hyperpigmentation, vitiligo, pale or jaundiced skin, and premature signs of aging like fine lines and wrinkles

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Cells were fixed with 4% PFA in PBS at room temperature for 15 min, permeabilized with 1% saponin and stained by rabbit anti-ACP1 antibody (LifeSpan Bioscience) followed by Alexa 488-conjugated goat anti-rabbit IgG antibody (Invitrogen)

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