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The Seahorse buffer for cells cultured in glucose-deprived medium consisted of glucose-free DMEM, phenol red, 2 mM sodium pyruvate, and 2 mM glutamine

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Bone marrow examination (rarely needed) showing erythroid hyperplasia

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Otherwise, bile duct-ligated rats commonly presented intracranial (superior sagittal sinus), portal and caval hypertension and aortal hypotension, gross brain swelling, hemorrhage and lesions, heart dysfunction, lung lesions, liver and kidney failure, gastrointestinal lesions, and severe arterial and venous thrombosis, peripherally and centrally