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293 Additionally, subsequent research in 2021 on glioblastoma stem-like cells (GSCs) showed that ES/Cu directly targets the mitochondrial membrane, inducing a significant increase in mitochondrial ROS, ultimately leading to non-apoptotic, copper-dependent cell death

10.1007/978-3-030-02254-9_1 Chaperokine activity of heat shock proteins

The cells were incubated with blocking buffer (1% (w/v) BSA, 22.52 mg/ml glycine, 0.1% (v/v) tween-20) for 30 mins and washed with PBS three times

Fakhri S, Yosifova Aneva I, Farzaei MH, Sobarzo-Snchez E (2019) The neuroprotective effects of astaxanthin: therapeutic targets and clinical perspective

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