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[PubMed ID: 7816935]

Vasquez-Dunddel D, Pan F, Zeng Q, Gorbounov M, Albesiano E, Fu J, Blosser RL, Tam AJ, Bruno T, Zhang H, Pardoll D, Kim Y

This compound is supplied for research purposes and is frequently incorporated into structured protocols exploring growth hormone signalling and anabolic support pathways

Cells were pelleted at 400x RCF for 5 min and frozen ready for protein purification

Colorimetric changes in the sample were detected using a microplate reader (BioTek Instruments, Inc., Winooski, UT, USA) at 532 nm

Funding This research has been supported by the National Natural Science Foundation of China (Grant No.: 81601943), and the China Hubei Provincial Health Commission Research Project (Grant No.: WJ2019M023)