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Samples were centrifuged at 15,000 rpm at 4 C for 10 min, and the supernatant was passed through a 0.45 m syringe filter (Fisher)

MORs act as autoreceptors, having direct effects and reducing AP firing within the recorded neuron, but can have similar effects in non-POMC neurons (Kelly et al., 1990, 1992

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[DOI] [PMC free article] [PubMed] [Google Scholar] 117.Jang M.-H., Bonaguidi M.A., Kitabatake Y., Sun J., Song J., Kang E., Jun H., Zhong C., Su Y., Guo J.U., et al
