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The reaction mixture is then centrifuged and washed until the precipitate is free from sulphates

Metals like iron and copper may induce oxidative damage by acting as catalysts for reactions that generate free radicals

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44 KristensssonE.SundqvistM.AstinM.KjerlingM.MattssonH.Dornonville de la CourC.et al (2006)

The method of calculating binding constants was to measure the absorbance (minus the absorbance of the buffer) of 100 L of each compound by itself diluted by 100 L of buffer at a given wavelength (in this case at 200 nm) at the range of concentrations utilized in the experiments