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153 Amongst these individuals, a greater incidence of erosive esophagitis is observed in those with neuropathy than amongst those without, despite both experiencing similar GORD symptoms

Other recognized motor signs and symptoms include gait and posture disturbances such as festination (rapid shuffling steps and a forward-flexed posture when walking), speech and swallowing disturbances including voice disorders, masklike face expression or small handwriting, although the range of possible motor problems that can appear is large

Once researchers have injected the correct amount of solvent, they can dispose of the needle and syringe in a sharps container

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Absorbance was measured at 450 nm (630 nm as reference wavelength) on a Clariostar plate reader (BMG Labtech)

Sigma) on ice for 20 min, and a nuclear run-on reaction was carried out at 30 C for 5 min in a reaction buffer [20 mM Tris pH 7.7, 200 mM KCl, 5 mM MgCl 2 , 1.25 mM dithiothreitol, 0.4 U/L SUPERase-In RNase Inhibitor (Fisher), 0.5% sarkosyl, 12.5 M biotin-11-CTP (Enzo Life Sciences), 12.5 M biotin-11-UTP (Biotium), 62.5 M ATP (Fisher), and 62.5 M GTP (Fisher)] to label newly transcribed RNA with biotin