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The coverslips containing the cells were then washed with ice-cold PBS three times, fixed with 4% paraformaldehyde at 4 C for 30 min, permeabilized with Triton X-100 for 10 min, and subjected to three 5-min washes

Role of tubulin-associated proteins in microtubule nucleation and elongation

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Afterward, visualization of the relevant results was accomplished via the Bioinformatics online platform
