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Description
Gorska, E., Popko, K., Stelmaszczyk-Emmel, A., Ciepiela, O., Kucharska, A., & Wasik, M
Cultured cells were stimulated for 6 h with PMA (50 ng ml 1 ) (BioGems) and ionomycin (1 g ml 1 ) (BioGems) in the presence of brefeldin A (1 g ml 1 ) (GolgiPlug, BD Biosciences), stained for viability (Zombie UV Fixable Viability Dye (BioLegend)) and surface markers, fixed and permeabilized with 3.7% formaldehyde (Sigma) and 0.1% NP-40, respectively, and stained for intracellular cytokines
Research support was provided by the Swiss National Science Foundation (SNSF) SPIRIT project (grant no
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BPC157 promotes cellular regeneration and tissue repair pathways
Cluster 2, Australia, Belgium, Brazil, Germany, Israel and Turkey
