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Description
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After treatment, tissues were maintained in 2% FBS DMEM until the 10th days, the medium was changed every 3 days
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Together with IL-12 and IL-18 or transforming tumor necrosis growth factor (TGF)-, IL-6, and IL-23, TNF- induces the differentiation of nave CD4+ T cells into pro-inflammatory T helper cells of type 1 (T H 1) and type 17 (T H 17), respectively [6,12,13]
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