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The expression of -smooth muscle actin (-SMA), as measured by immunostaining and Western blotting, was used as a marker of PSC activation

11 Liver tumors cause damage to the liver by several mechanisms: inflammatory effects, obstruction of the biliary system, obstruction of the vascular compartment or adjacent organs, and spontaneous rupture with hemoabdomen

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as well as non-HC genetic iron overload (ferroportin disease, hereditary aceruloplasminemia) should also be excluded

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