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5000 cells/well were seeded in cell impedance measurement plates (ACEA Biosciences, San Diego, US) and cell impedance was continuously determined using the xCELLigence Real Time Cell Analyzer system (OLS OMNI Life Science, Bremen, Germany)

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The innate immune system recognizes the conserved structures of pathogens via a series of pattern recognition receptors (PRRs), prompting immune cells to respond and secrete inflammatory cytokines (Rock et al., 2010