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Supernatants were directly diluted 1:2 in LDH storage buffer containing 200 mM Tris (Carl Roth)-HCL (VWR International, Darmstadt, Germany) at pH 7.5, 10% glycerol (Sigma-Aldrich), 1% bovine serum albumin (Sigma-Aldrich) and AQUA AD iniectabilia to conserve LDH activity during freezing

Aliquots of 50 l of the incubation sample mixture were collected at 0, 5, 10, 15, 30, and 45 min

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