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The cultures were subsequently normalized to a concentration of 1 10 8 CFU/mL for subsequent experiments, ensuring representation of peak metabolic activity under standard conditions

Additionally, when considering cognitive decline caused by aging, dietary intake of caffeine can protect the brain by regulating the Bax/Bcl2 ratio, caspase-3, and PARP-1 levels to reduce oxidative stress, and COX-2, NOS-2, TNF, and IL-1 to further reduce D-galactose-induced neuroinflammation and neurodegeneration (Ullah et al., 2015)

GLP-1 secretion induced by Gs activation with100-M DCA or 10-mM L-glutamine were further enhanced by high glucose (25 mM) stimulation (Fig