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2.7 Aortic ring assay The mouse aortic ring assay was conducted according to a previously described method ( 2.8 Western blotting Proteins were extracted from the tissue samples, C2C12 myocytes, or HUVECs using RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Nacalai Tesque, Kyoto, Japan), separated using SDS-PAGE, and transferred to PVDF membranes

doi: 10.2217/bmm.13.158

Burtle, G

Any metabolic changes evident from metabolomics analysis in dogs with diet-associated DCM must be differentiated from those occurring in dogs with primary DCM

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