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Deficiency of a transmembrane prolyl 4-hydroxylase in the zebrafish leads to basement membrane defects and compromised kidney function

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Group 2 samples were immediately fixed in 4% paraformaldehyde and then embedded in paraffin for immunohistochemical analysis

(American Chemical Society)Methods for accurately quantifying the concn

We've found that the reasoning boils down to three core pillars

Subsequently, the absorbance of supernatants in each well was measured at 450 nm using a microplate reader (Model 680