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This refinement involves the emergence of a second transient transcriptional program that first turns on (increase in PC2 genes) and then off during melanoma (and melanocyte) differentiation transitions (Figures 1C and 1E), and is thus reminiscent of other transient developmental transcriptional programs (Spitz and Furlong, 2012

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Data for phenotypic analysis (e.g., survival assays, lifespan assays, egg retention assays, qRT-PCR measurements, and individual animal fluorescence measurements) were analyzed using GraphPad Prism 9.3.0